25-Hydroxyvitamin D

Alternative Names/Synonyms	25-OH Vitamin D, Calcidiol
Laboratory	Core Laboratory & Transfusion Medicine
Sub-section	Biochemistry
Specimen Type	Blood
Test Code	OHIP-Funded: 25HD Non-Funded: 25DS

Clinical Significance (If applicable)

Assisting in the diagnosis of severe vitamin D deficiency and in assessing status in patients with osteoporosis, inflammatory bowel disease, celiac disease, kidney disease, liver disease and pancreatitis.

Specimen Requirements (Sample Type)

Serum

Specimen Requirements (Volume (mL))

0.5 mL

Specimen Requirements (Tube Type)

Serum (Gold SST)
Collection Containers – Tube Types

Collection Requirements

Collection Instructions

Sample Stability

Room Temperature: 7 days

Fridge: 1 month @ 2-8°C

Freezer: 1 year @ -20°C

Cause for Rejection

Specimen Acceptability and Transport Guidance

Instrument, Methodology

Liaison XL, immunoassay

Test Availability

Weekdays

Routine Turnaround Time

3 days

Reference Ranges

Deficient: < 25 nmol/L
Insufficient: < 40 nmol/L
Desirable: > 75 nmol/L
Physiologic Range from UV Exposure: < 225 nmol/L
Excess Vitamin D Intake: > 600 nmol/L

In case of discrepancies, always interpret results using reference ranges as stated on laboratory reports and/or electronic patient chart.

Laboratory Contact Information

416-586-4800 extension 4688