25-Hydroxyvitamin D

Alternative Names/Synonyms	25-OH Vitamin D, Calcidiol
Laboratory	Core Laboratory & Transfusion Medicine
Sub-section	Biochemistry
Specimen Type	Blood
Test Code	OHIP-Funded: 25HD Non-Funded: 25DS

Clinical Significance

Assisting in the diagnosis of severe vitamin D deficiency and in assessing status in patients with osteoporosis, inflammatory bowel disease, celiac disease, kidney disease, liver disease and pancreatitis.

Specimen Requirements (Sample Type)

Serum₂

Specimen Requirements (Volume (mL))

0.5 mL

Specimen Requirements (Tube Type)

Serum (Gold SST)
Collection Containers – Tube Types

Collection Requirements

Blood Collection Instructions

Sample Stability

Room Temperature: 7 days

Fridge: 1 month @ 2-8°C

Freezer: 1 year @ -20°C

Cause for Rejection

Specimen Acceptability and Transport Guidance

Instrument, Methodology

Liaison XL, immunoassay

Test Availability

Weekdays

Routine Turnaround Time

3 days

Reference Ranges

Deficient: < 25 nmol/L
Insufficient: < 40 nmol/L
Desirable: > 75 nmol/L
Physiologic Range from UV Exposure: < 225 nmol/L
Excess Vitamin D Intake: > 600 nmol/L

In case of discrepancies, always interpret results using reference ranges as stated on laboratory reports and/or electronic patient chart.

Laboratory Contact Information

416-586-4800 extension 4688