Acanthamoeba

Detection of Acanthamoeba parasite
Microbiology
Bacteriology
Corneal scrapings Contact lens cases and fluid CSF (cerebrospinal fluid)

○ Corneal scrapings: sterile container with normal saline
○ Contact lens cases/fluid or CSF: sterile container


Corneal scrapings:
○ Collect scrapings from lesions or ulcers using a sterile spatula in normal saline (min volume 1 mL)

Contact lens cases and solution:
○ Collect 1 mL of contact lens solution in sterile container
○ Alternatively, both the lens case and contact lens solution bottle can be sent to the laboratory as a whole in a specimen bag

CSF:
○ Collect CSF by lumbar puncture in sterile container (min volume 1 mL)


Refer to section Sample Processing / Delivery

PHOL

Up to 3 days from receipt by PHOL
CSF specimens up to 1 day upon receipt by PHOL

416-586-4800 extension 4432


NO

○ Label specimen container. Place specimens in biohazard bag and seal.

○ Transport specimens to the UHN/SH Microbiology Laboratory ASAP at room temperature.

○ UHN/SH Microbiology Laboratory will refer all specimens to Public Health Ontario Laboratory (PHOL) for testing