○ Collect urine into a clean, sterile container, then transfer it to a BD grey top urine vacutainer tube. A sterile container is also acceptable. Transport to the microbiology laboratory ASAP after collection.
Midstream urine (MSU):
○ Clean urethral meatus. Void and discard the first 10 - 20 mL of urine in order to clear the urethra. Collect the subsequent urine into a clean, sterile container.
Neonatal bagged urine:
○ Place a clean collection bag over the external genitalia. Transfer the urine from the bag into a clean, sterile container.
Indwelling catheter (Foley catheter) urine:
○ Puncture catheter tubing aseptically and transfer the urine into a clean, sterile container.
Ileal conduit urine:
○ Clean the stomal opening with alcohol. Insert a sterile catheter to collect the urine and transfer the urine into a clean, sterile container.
In and Out catheter / catheter insertion urine:
○ Collect urine into a clean, sterile container immediately following the initial insertion of an indwelling catheter into the bladder.
Nephrostomy urine
○ Urine draining from a nephrostomy tube placed in the renal pelvis is collected into a clean, sterile container.
Bladder / Cystoscopy urine
○ Collect urine into a clean, sterile container following temporary insertion of a sterile catheter or cystoscope into the bladder.
Suprapubic urine aspirate
○ Aspirate urine through the bladder using a sterile needle and syringe. Transfer the urine into a clean, sterile container.
Segmented urine
○ Collect these specimens for the diagnosis of chronic bacterial prostatitis. Collect three urines plus prostatic secretions (after massaging prostate through the rectum) and designate them as follows:
VB1 = first voided urine representing the urethra
VB2 = midstream urine representing the bladder
VB3 = first voided urine after prostatic massage representing the prostate
EPS = expressed prostatic secretions