Bacterial Culture & Susceptibility – Bone Marrow
| C&S Bone Marrow | |
| Microbiology | |
| Bacteriology | |
| ○ Bone marrow aspirate ○ Bone marrow biopsy |
Culture-based detection of bacterial pathogens from bone marrow
○ Bone marrow aspirate: pediatric blood culture bottle and anaerobic blood culture bottle or SPS tube
○ Bone marrow biopsy: sterile container
○ Bone marrow biopsy: sterile container
Bone marrow aspirate
○ Collect bone marrow aspirates using a sterile needle and dispense into pediatric blood culture bottle (min volume 0.5 mL and preferably 3mL) and same volume into anaerobic blood culture bottle or SPS tube.
Bone marrow biopsy
○ Collect bone marrow biopsy and place in clean, sterile container, on a sterile saline soaked gauze.
○ Collect bone marrow aspirates using a sterile needle and dispense into pediatric blood culture bottle (min volume 0.5 mL and preferably 3mL) and same volume into anaerobic blood culture bottle or SPS tube.
Bone marrow biopsy
○ Collect bone marrow biopsy and place in clean, sterile container, on a sterile saline soaked gauze.
Refer to section Sample Processing / Delivery
In-house
○ Up to 7 days
416-586-4800 extension 4432
NO
○ Label specimen container and outer biohazard specimen bag with printed electronic laboratory order sticker. Place specimens in biohazard bag, seal
○ Transport specimens to the UHN/SH Microbiology Laboratory ASAP at refrigeration temperature (2-8˚C) if a delay of more than 2h is expected in transport. The blood culture bottles should be stored at room temperature (20-25 ˚C) even if a delay is expected.
○ Transport specimens to the UHN/SH Microbiology Laboratory ASAP at refrigeration temperature (2-8˚C) if a delay of more than 2h is expected in transport. The blood culture bottles should be stored at room temperature (20-25 ˚C) even if a delay is expected.