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Breast Tumour Biomarkers, ER, PR , HER2

Alternative Names/Synonyms	Solid Tumour Biomarker Testing, ER, PR , HER2, IHC, ISH
Laboratory	Anatomical Pathology
Sub-section	Surgical Pathology

Ordering Instructions, Requisition

Pathology Consultation and Biomarker Requisition

Other Information

Breast specimens must have a cold ischemic time of <60 minutes proceeded by fixation in 10% neutral buffered formalin for 6-72 hours. (as per CAP/ASCO guidelines) Microwave processed and decalcified samples are not suitable for testing. Mercurochrome use as a dye marker is not recommended.

Clinical Significance

Reflex testing on all newly diagnosed breast cancers.
Biomarkers: ER, PR , HER2 IHC/ISH

Specimen Requirements (Sample Type)

Formalin fixed paraffin embedded block or tissue sections cut at 4 microns mounted on "Superfrost Plus" charged slides.

Cause for Rejection

Specimen Acceptability and Transport Guidance

Instrument, Methodology

Dako Omnis

Test Availability

Monday to Friday; 8 am to 5 pm

Reference Ranges

Please refer directly to the Pathology Report

Laboratory Contact Information

Tel: (416) 586-4800 extension 4457
Fax: (416) 586-8628
Email: PLMconsults@sinaihealth.ca

References

https://www.cancercareontario.ca/en/guidelines-advice/treatment-modality/pathology-laboratory-testing/genetic-testing-resources