Breast specimens must have a cold ischemic time of <60 minutes proceeded by fixation in 10% neutral buffered formalin for 6-72 hours. (as per CAP/ASCO guidelines) Microwave processed and decalcified samples are not suitable for testing. Mercurochrome use as a dye marker is not recommended.
Testing on locally recurrent unresectable or metastatic triple negative breast cancer (TNBC)
Formalin fixed paraffin embedded block or tissue sections cut at 4 microns mounted on "Superfrost Plus" charged slides.