CMV, cytomegalovirus - detection

BAL:
○ Pass bronchoscope transnasally or transorally (if patient is not intubated) or via endotracheal tube (if patient is intubated). Instill a large volume of 0.85% sterile saline (nonbacteriostatic) into the airway lumen. Discard the initial fluid recovered (likely contaminated). Collect remaining fluid in a clean, sterile container via suction.

Stool
○ Pass stool into a clean, dry pan or container mounted on the toilet. Collect at least 5 mL of watery diarrheal stool (10 to 30 mL preferred), approximately 1 gram of fecal material or a walnut-sized portion of stool or a sufficient amount of stool to the line of the transport container. Transport to the laboratory immediately after collection

Amniotic fluid:
○ Decontaminate skin, insert needle into uterus aseptically and collect amniotic fluid via percutaneous aspiration (amniocentesis). Collect fluid in sterile container

Bile
○ Bile may be aspirated with a syringe during surgery or collected in a sterile container from a post-op drain

CSF:
○ Decontaminate skin, collect at least 0,5mL of CSF

Sterile fluids/aspirates
○ Decontaminate skin with 70% alcohol followed by iodine or chlorhexidine. Aspirate fluid by percutaneous aspiration using aseptic technique and a syringe and needle.
○ Collect bone marrow aspirates using a sterile needle and dispense into a clean sterile container containing sodium heparin (min volume 1 mL)

Ocular fluid
○ Collect an aspirate of vitreous fluid or paracentesis of anterior chamber using a needle aspiration technique. Dispense fluid into sterile container.

Urine:
Midstream urine (MSU):
○ Clean urethral meatus. Void and discard the first 10 - 20 mL of urine in order to clear the urethra. Collect the subsequent urine into a clean, sterile container.
○ Neonatal urine: 3-4 ml to be collected

Neonatal surveillance swab:
○ Swab should be taken from the throat of neonate and placed in transport medium.

Tissue
○ Collect 5-10 mm3 tissue aseptically using surgical technique/punch biopsy. Place in a clean, sterile container with a small amount of sterile saline. Do not wrap tissue in gauze.
○ If lesion for biopsy is cutaneous, collect biopsy from periphery of lesion or aspirate material under lesion margin

Whole blood/plasma
○ Decontaminate skin with 70% alcohol followed by iodine or chlorhexidine, collect at least 5 mL of blood
○ Neonatal blood (min 1 ml)