CMV, cytomegalovirus - drug resistance genotyping

Alternative Names/Synonyms	Molecular detection of drug resistance in human cytomegalovirus (CMV)
Laboratory	Microbiology
Sub-section	Virology
Specimen Type	○ Whole blood ○ Plasma

Other Information

○ Specimens submitted for genotyping must have a positive PCR result indicating the presence of CMV DNA

○ Testing will only be performed when anti-viral resistant CMV is suspected. This is characterized by a lack of improvement (or relapse) in CMV viremia or clinical disease during antiviral therapy (for > 6 weeks)
○ Upon special request, region of UL56 and UL89 can be assessed for Letermovir resistance.

Specimen Requirements (Tube Type)

○ EDTA tube (lavender or pink top) (min volume 5 mL)

Collection Requirements

○ Decontaminate skin with 70% alcohol followed by iodine or chlorhexidine, collect at least 5 mL of blood

Sample Stability

Refer to section Sample Processing / Delivery

Referral Laboratory

NML

Routine Turnaround Time

○ 10 days upon receipt by NML

Laboratory Contact Information

416-586-4800 extension 4482

Approval Required

YES

Sample Processing / Delivery

○ Label specimen container. Place specimens in biohazard bag and seal.

○ Specimens should be stored at refrigeration temperature (2-8˚C) after collection and transported to the UHN/SH Microbiology Laboratory ASAP

○ UHN/SH Microbiology Laboratory will refer the original positive specimen to the National Microbiology Laboratory for testing upon request