Coccidiodes (Please notify laboratory if suspected)
| Detection of Coccidioides species (Coccidiomycosis) | |
| Microbiology | |
| Mycology | |
| Fungal culture: ○ Blood ○ Tissue/biopsy ○ BAL ○ Any other specimen submitted for routine fungal culture Antigen detection: ○ Plasma, serum ○ BAL, CSF, other body fluids ○ Urine |
Other Information
○ Ensure that the laboratory is notified if this agent is suspected
○ For Coccidioides serology, see Fungal serology
○ For Coccidioides serology, see Fungal serology
Fungal culture:
○ Blood: SPS vacutainer tube
○ BAL or Tissue/biopsy: sterile container
Antigen detection
○ Serum: serum separator tube or red top tube (min volume 5mL)
○ Plasma: EDTA, heparin or sodium citrate tube (5 mL)
○ Urine: sterile container (0,5 mL)
○ BAL/other body fluids: sterile container (0,5mL)
○ CSF: sterile container (0,8 mL)
○ Blood: SPS vacutainer tube
○ BAL or Tissue/biopsy: sterile container
Antigen detection
○ Serum: serum separator tube or red top tube (min volume 5mL)
○ Plasma: EDTA, heparin or sodium citrate tube (5 mL)
○ Urine: sterile container (0,5 mL)
○ BAL/other body fluids: sterile container (0,5mL)
○ CSF: sterile container (0,8 mL)
Fungal culture:
○ Blood: SPS vacutainer tube
○ BAL or Tissue/biopsy: sterile container
Antigen detection
○ Serum: serum separator tube or red top tube (min volume 5mL)
○ Plasma: EDTA, heparin or sodium citrate tube (5 mL)
○ Urine: sterile container (0,5 mL)
○ BAL/other body fluids: sterile container (0,5mL)
○ CSF: sterile container (0,8 mL)
○ Blood: SPS vacutainer tube
○ BAL or Tissue/biopsy: sterile container
Antigen detection
○ Serum: serum separator tube or red top tube (min volume 5mL)
○ Plasma: EDTA, heparin or sodium citrate tube (5 mL)
○ Urine: sterile container (0,5 mL)
○ BAL/other body fluids: sterile container (0,5mL)
○ CSF: sterile container (0,8 mL)
Refer to section Sample Processing / Delivery
Culture In-house Quantitative Antigen EIA
MIRAVISTA
MIRAVISTA
○ Culture/confirmation: Up to 21 days
○ Antigen detection: Up to 3 days after receipt of specimen
○ Antigen detection: Up to 3 days after receipt of specimen
416-586-4800 extension 4432
YES; laboratory should be notified if this agent is suspected
○ Label specimen container and place in biohazard bag and seal.
○ Blood, CSF and skin should be stored at room temp (20-25˚C) after collection and submitted to the UHN/SH Microbiology Laboratory ASAP. Other specimens should be stored at refrigeration temp if a delay of more than 2h is expected.
○ The specimen will be referred to the PHOL for testing if fungal growth is suggestive of Coccidioides species.
○ The specimen will be referred to MiraVista Diagnostics for antigen detection testing.
○ Blood, CSF and skin should be stored at room temp (20-25˚C) after collection and submitted to the UHN/SH Microbiology Laboratory ASAP. Other specimens should be stored at refrigeration temp if a delay of more than 2h is expected.
○ The specimen will be referred to the PHOL for testing if fungal growth is suggestive of Coccidioides species.
○ The specimen will be referred to MiraVista Diagnostics for antigen detection testing.