Cryptosporidium

Detection of Cryptosporidium parasite species
Microbiology
Bacteriology
○ Stool ○ Duodenal contents ○ Tissue/biopsy materials
Other Information
○ Collect 1 specimen per day (1 Yellow top and 1 White top container) for 3 days in each vial for a total of six (6) specimens per patient with suspected parasitic infection.

○ Ensure patient avoids anti-diarrheal medications, radiological dye (barium) and antibiotics prior to producing the specimen as they may interfere with parasite detection.

○ Please provide appropriate clinical information on the requisition (e.g. travel history or country of origin if refugee, returned traveller or recent immigrant, consumption of certain food products, eosinophilia, HIV, immunocompromise, critical illness, admitted to ICU or previous parasite infection
○ Duodenal contents/tissues and biopsies are only acceptable specimens if the initial stool specimens are negative.
○ Stool: Parasitology Kit (3 each of SAF yellow top vial, white top sterile vial)
○ Duodenal contents/tissue/biopsy material: sterile container (with small amount of saline ~ 1mL)

Stool
○ Collect stool immediately after bowel movement in a dry, clean container (e.g. Chamber pot, wide-mouth jar, pie plate, newspaper, or saran wrap on toilet seat). Follow instructions below to add specimen to BOTH yellow and white capped containers. Specimen should not be contaminated with urine or toilet water.
Container 1 SAF (Yellow top) vial:
○ Use plastic spork attached to lid to add sample (from beginning, middle and end of stool) to yellow capped bottle so that the preservative fluid level is approximately ¾ full. Mix thoroughly by shaking. Ratio should be 1 part solid stool to 3 parts SAF or 1 part liquid faeces to 1 part SAF.

Container 2 Sterile (White top) vial:
○ Using plastic spork, Add stool directly to sterile white capped container.
Duodenal contents
○ If specimen is collected by duodenal aspiration, retrieve fluid from the duodenum via nasogastric intubation and place in sterile container.
○ If specimen collected by Entero-Test capsule, upon retrieval of string, specimen should be placed in sterile container with ~ 1mL of saline.

Tissue/biopsy materials
○ Collect 5-10 mm3 tissue aseptically using surgical technique/punch biopsy. Place in a clean, sterile container with a small amount of sterile saline. Do not wrap tissue in gauze.

Refer to section Sample Processing / Delivery
PHOL
○ Up to 5 days from receipt at PHOL
416-586-4800 extension 4479
NO
○ Label specimen container. Place specimens in biohazard bag and seal.

○ Specimens should be stored at room temperature (20-25˚C) and transported to the UHN/SH Microbiology Laboratory ASAP after collection. For stool, keep Yellow top tube (SAF vial) at room temperature while the White-top vial should be refrigerated (2-8˚C) prior to transport.

○ The specimen will be referred to the Public Health Ontario Laboratory for testing.