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Direct Antiglobulin Test
Alternative Names/Synonyms
DAT, Direct Coombs Test
Laboratory
Core Laboratory & Transfusion Medicine
Sub-section
Transfusion Medicine
Specimen Type
Blood
Test Code
DAT1 - For Adult
DAT2 For Neonate
Ordering Instructions, Requisition
MS 327 Blood Transfusion Product Request
Clinical Significance (If applicable)
To determine whether patient's red blood cells have been sensitized in vivo with immunoglobulin, complement or both
Specimen Requirements (Sample Type)
Peripheral blood
Specimen Requirements (Volume (mL))
6 mL
Specimen Requirements (Tube Type)
1 Pink top K2EDTA
Collection Containers – Tube Types
Collection Requirements
Collect 1 Pink top tube EDTA. Mix well at time of collection
Collection Instructions
Storage / Shipment Details
Send ASAP to Core Laboratory
Sample Stability
Stable for 21 days at 1-6C
Cause for Rejection
Specimen Acceptability and Transport Guidance
Test Availability
24 hours / 7 days
STAT Turnaround Time
30 mins. Longer if result is positive
Routine Turnaround Time
30 minutes
Laboratory Contact Information
Tel #: 416-586-4502
Fax #: 416-586-8504
Sample Processing / Delivery
Please deliver to the Core Laboratory, 11th Floor
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