Galactomannan (Aspergillus) - detection
| Serologic detection of the Aspergillus galactomannan antigen | |
| Microbiology | |
| Serology | |
| ○ BAL ○ Serum |
Other Information
○ BAL and serum samples must be uncontaminated with fungal spores and/or bacteria
○ BAL: sterile container
○ Serum: serum separator tube (min volume 5 mL blood)
○ Serum: serum separator tube (min volume 5 mL blood)
BAL:
○ Pass bronchoscope transnasally or transorally (if patient is not intubated) or via endotracheal tube (if patient is intubated). Instill a large volume of 0.85% sterile saline (nonbacteriostatic) into the airway lumen. Discard the initial fluid recovered (likely contaminated). Collect remaining fluid in a clean, sterile container via suction.
Serum:
○ Decontaminate skin with 70% alcohol followed by iodine or chlorhexidine, collect at least 5 mL of blood if multiple tests will be ordered
○ Pass bronchoscope transnasally or transorally (if patient is not intubated) or via endotracheal tube (if patient is intubated). Instill a large volume of 0.85% sterile saline (nonbacteriostatic) into the airway lumen. Discard the initial fluid recovered (likely contaminated). Collect remaining fluid in a clean, sterile container via suction.
Serum:
○ Decontaminate skin with 70% alcohol followed by iodine or chlorhexidine, collect at least 5 mL of blood if multiple tests will be ordered
Refer to section Sample Processing / Delivery
In-house
○ Up to 3 days
416-586-4800 extension 4432
NO
○ Label specimen container. Place specimens in biohazard bag and seal.
○ Specimens should be stored at room temperature (20-25˚C) after collection and transported to the UHN/SH Microbiology Laboratory ASAP.
○ Specimens should be stored at room temperature (20-25˚C) after collection and transported to the UHN/SH Microbiology Laboratory ASAP.