Haemophilus ducreyi

Alternative Names/Synonyms	Molecular detection of Haemophilus ducreyi (chancroid)
Laboratory	Microbiology
Sub-section	Bacteriology
Specimen Type	○ Bubo aspirate ○ Genital ulcer swab

Specimen Requirements (Tube Type)

○ Bubo aspirate: sterile container (1mL)
○ Genital ulcer swab: Dacron swab in Universal Transport Medium (1mL of fluid) or dry

Collection Requirements

Bubo aspirate:
○ Using a needle and syringe aspirate pustular material from the bubo through normal tissue. Collect specimen in sterile container.

Genital ulcer swab:
○ Cleanse the genital area by flushing with sterile physiological saline. Swab the base of the genital ulcer using a Dacron or cotton swab to obtain specimens from ulcers.

Sample Stability

Refer to section Sample Processing / Delivery

Referral Laboratory

PHOL

Routine Turnaround Time

○ Up to 28 days from receipt of specimen at PHO laboratory

Laboratory Contact Information

416-586-4800 extension 4508

Approval Required

Sample Processing / Delivery

○ Label specimen container. Place specimens in biohazard bag and seal.

○ Specimens should be stored and transported to the UHN/SH Microbiology Laboratory frozen (-20˚C) after collection. If the specimen can’t be frozen, store at refrigeration temperature (2-8˚C) and transport ASAP to Microbiology Laboratory.

○ The specimen will be referred to the Public Health Ontario Laboratory for testing