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Hereditary Spherocytosis Screening

Alternative Names/Synonyms	EMA binding, Eosin-5-Maleimide
Laboratory	Core Laboratory & Transfusion Medicine
Sub-section	Flow Cytometry
Specimen Type	Blood
Test Code	OF1

Ordering Instructions, Requisition

Mount Sinai Hospital -Special Laboratory Requisition Report Form MS 331

Other Information

The term "Osmotic Fragility" may be requested by the physician. They should be notified that OF is no longer available. And that the alternative test is Hereditary spherocytosis screening by Flow Cytometry. Other name of test for flow are EMA binding and Eosin-5-Maleimide

Specimen Requirements (Sample Type)

Blood

Specimen Requirements (Volume (mL))

Optimum full draw

Specimen Requirements (Tube Type)

2 mL EDTA Lavender Tube or 500 uL EDTA microcontainer
Collection Containers – Tube Types

Collection Requirements

Blood Collection Instructions

Storage / Shipment Details

Keep blood at room temperature

Sample Stability

Sample must be fresh (taken maximun 24 hours before testing)

Cause for Rejection

Specimens that cannot be tested within 24 hours from collection time. Do not refridgerate.
Specimen Acceptability and Transport Guidance

Instrument, Methodology

Hospital for Sick Children Flow Cytometry

Test Availability

Samples to be received in the lab from Monday to Wednesday and no later than 12:00pm on Wednesday, excluding holidays and weekends.

Routine Turnaround Time

Three working days

Laboratory Contact Information

416-586-4800 Extension 8519

Special Instructions

A complete blood count (CBC) and morphology are required at the time of draw of the specimen.