Immunodeficiency Panel

Alternative Names/Synonyms	CD3/4/8, T Cell Subset, T4/T8 Ratio, CD4/8, Blood Lymphocyte Subset
Laboratory	Core Laboratory & Transfusion Medicine
Sub-section	Flow Cytometry
Specimen Type	Blood
Test Code	TCD48

Ordering Instructions, Requisition

Mount Sinai Hospital -Special Laboratory Requisition Report Form MS 331, Womens College Hospital -Flow Cytometry Requisition Womens College Hospital, Markham Stouffville Hospital (Oak Valley Health) -Flow Cytometry Requisition Markham Stouffville

Clinical Significance (If applicable)

Test is used when clinical information suggests a T-cell lymphocyte population abnormality. T lymph percentages and absoute counts may be used to evaluate immune competency

Specimen Requirements (Sample Type)

Whole Blood

Specimen Requirements (Volume (mL))

Optimum full draw

Specimen Requirements (Tube Type)

2 x EDTA Lavender Tube
Collection Containers – Tube Types

Collection Requirements

Collection Instructions

Storage / Shipment Details

Room temperature

Sample Stability

To be processed within 24 hours of collection

Cause for Rejection

Specimens that have been refrigerated, severely hemolysed, clotted, under-filled, not maintained at room temperature, and greater than 24 hours old.
Specimen Acceptability and Transport Guidance

Instrument, Methodology

Beckman Coulter Navios Ex

Test Availability

Monday to Friday and no later than 3 pm on Fridays. No Flow Cytometry testing on weekends and statutory holidays. Specimen to be stored at room temperature.

Routine Turnaround Time

3 days

Reference Ranges

Percent
CD3 57.9 – 87.1 %
CD3/CD4 28.4 – 64.6 %
CD3/CD8 11.4 – 40.1 %

Absolute
CD3 0.670 – 2.290 x10*9/L
CD3/CD4 0.430 – 1.690 x10*9/L
CD3/CD8 0.150 – 1.070 x10*9/L
CD4/CD8 Ratio 0.900 – 3.700
In case of discrepancies, always interpret results using reference ranges as stated on laboratory reports and/or electronic patient chart.

Laboratory Contact Information

416-586-4800 Extension 8519