Japanese Encephalitis B virus

Alternative Names/Synonyms	Detection of Japanese Encephalitis B virus nucleic acids or antibodies
Laboratory	Microbiology
Sub-section	Virology
Specimen Type	○ Whole blood ○ Serum ○ EDTA-blood/plasma (PCR) ○ CSF (PCR)

Other Information

○ If submitting a CSF, a paired serum specimen must also be submitted.
○ To demonstrate seroconversion, both acute and convalescent samples must be tested in parallel.

Specimen Requirements (Tube Type)

○ Whole blood/serum: serum separator tube (clotted blood)
○ EDTA-blood/plasma: Vacutainer tube (lavender top)
○ CSF: sterile container

Collection Requirements

Whole blood/serum/plasma:
○ Decontaminate skin with 70% alcohol followed by iodine or chlorhexidine, collect at least 5 mL of blood
CSF:
○ Decontaminate skin, collect at least 0.5 mL of CSF by lumbar puncture

Sample Stability

Refer to section Sample Processing / Delivery

Referral Laboratory

PHOL

Routine Turnaround Time

○ Up to 28 days upon receipt from PHO laboratory

Laboratory Contact Information

416-586-4800 extension 4546

Approval Required

YES for molecular testing

Sample Processing / Delivery

○ Label specimen container. Place specimens in biohazard bag and seal.

○ Specimens should be stored and transported to the UHN/SH Microbiology Laboratory at refrigeration temperature (2-8˚C) immediately after collection

○ The specimen will be referred to the Public Health Ontario Laboratory for testing. Samples will be referred to NML.