○ LCMV is a Risk Group 3 pathogen and PHOL will not perform other testing until LCMV is ruled out by NML. If further testing is required, submit separate specimens and notify laboratory staff that the patient is under investigation for LCMV.
○ Serology: Serum separator tube (clotted blood) or red top tube (min volume 5 mL) ○ PCR: whole blood or serum: Serum separator tube, EDTA, heparin or citrate tubes (min volume 5 mL) ○ CSF: sterile container (min volume 1.5-2mL) ○ Tissues: sterile container with a small amount of saline (min 0.5 mL)
○ Decontaminate skin with 70% alcohol followed by iodine or chlorhexidine, collect at least 5 mL of blood Tissue/biopsy ○ Collect 5-10 mm3 tissue aseptically using surgical technique/punch biopsy. Place in a clean, sterile container with a small amount of sterile saline. CSF ○ Decontaminate skin, collect at least 1.5-2 mL of CSF by lumbar puncture.
Refer to section Sample Processing / Delivery
PHOL
○ 28 days
416-586-4800 extension 4552
YES
○ Label specimen container. Place specimens in biohazard bag and seal.
○ Specimens should be stored and transported to the UHN/SH Microbiology Laboratory at refrigeration temperature (2-8˚C) immediately after collection. Formalin-fixed specimens can be sent at room temperature.
○ The specimen will be referred to the Public Health Ontario Laboratory for testing. PHOL will refer the specimen to NML.