Parasites (other) – Anisakis

Detection of Anisakis (roundworm)
Microbiology
Bacteriology
○ Biopsy material ○ Worm removed from stomach during endoscopy
Other Information
○ Indicate appropriate clinical information on requisition (e.g. symptoms, date of onset, consumption of undercooked or raw seafood, travel history or country of origin if refugee, returned traveller or recent immigrant, eosinophilia, HIV, immunocompromised, critical illness or ICU admission or previous parasite infection.
○ Sterile container (plus small amount of saline if biopsy material)
Biopsy material
○ Collect at least 5-10 mm3 tissue aseptically using surgical technique. Place in a clean, sterile container with a small amount of sterile saline.

Worm removed during endoscopy
○ Collect worm aseptically and place in a clean, sterile container.

Refer to section Sample Processing / Delivery
PHOL
○ Up to 3 days from receipt at PHO Laboratory
416-586-4800 extension 4583
NO
○ Label specimen container. Place specimens in biohazard bag and seal.

○ Specimens should be stored and transported to the UHN/SH Microbiology Laboratory at room temperature (20-25˚C) ASAP after collection.

○ The specimen will be referred to Public Health Ontario Laboratory for testing