○ Decontaminate skin with 70% alcohol followed by iodine or chlorhexidine, collect at least 5 mL of blood into appropriate container
○ CSF by lumbar puncture: Decontaminate skin, collect at least 1mL of CSF by lumbar puncture into a sterile container; collect more CSF if additional cultures are requested (AFB, fungal, etc.). If more than one tube can be successfully collected, the microbiology laboratory should receive the second or third tubes (whichever is less bloody) to avoid contamination by skin flora or blood.