Parasites (other) – Paragonimus
| Serologic and/or microscopic identification of Paragonimus species (intestinal/lung fluke) | |
| Microbiology | |
| Parasitology | |
| Microscopy ○ Stool ○ Sputum Serology ○ Whole blood ○ Serum |
Other Information
Stool
○ Collect 1 specimen per day (1 Yellow top and 1 White top container) for 3 days in each vial for a total of six (6) specimens per patient with suspected parasitic infection.
○ Ensure patient avoids anti-diarrheal medications, radiological dye (barium) and antibiotics prior to producing the specimen as they may interfere with parasite detection.
All specimens
○ Please provide appropriate clinical information on the requisition (e.g. travel history or country of origin if refugee, returned traveller or recent immigrant, consumption of certain food products, eosinophilia, HIV, immunocompromise, critical illness, admitted to ICU or previous parasite infection
Serology
○An acute and convalescent may be required for laboratory diagnosis.
○ Collect 1 specimen per day (1 Yellow top and 1 White top container) for 3 days in each vial for a total of six (6) specimens per patient with suspected parasitic infection.
○ Ensure patient avoids anti-diarrheal medications, radiological dye (barium) and antibiotics prior to producing the specimen as they may interfere with parasite detection.
All specimens
○ Please provide appropriate clinical information on the requisition (e.g. travel history or country of origin if refugee, returned traveller or recent immigrant, consumption of certain food products, eosinophilia, HIV, immunocompromise, critical illness, admitted to ICU or previous parasite infection
Serology
○An acute and convalescent may be required for laboratory diagnosis.
○ Stool: Parasitology Kit (3 each of SAF yellow top vial, white top sterile vial)
○ Sputum: sterile container
○ Whole blood/serum: serum separator tube (clotted blood)
○ Sputum: sterile container
○ Whole blood/serum: serum separator tube (clotted blood)
Stool
○ Collect stool immediately after bowel movement in a dry, clean container (e.g. Chamber pot, wide-mouth jar, pie plate, newspaper, or saran wrap on toilet seat). Follow instructions below to add specimen to BOTH yellow and white capped containers. Specimen should not be contaminated with urine or toilet water.
Container 1 SAF (Yellow top) vial:
○ Use plastic spork attached to lid to add sample (from beginning, middle and end of stool) to yellow capped bottle so that the preservative fluid level is approximately ¾ full. Mix thoroughly by shaking. Ratio should be 1 part solid stool to 3 parts SAF or 1 part liquid faeces to 1 part SAF.
Container 2 Sterile (White top) vial:
○ Using plastic spork, Add stool directly to sterile white capped container.
Sputum
○ Collect sputum by expectoration or by tracheal aspiration in sterile container.
Whole blood/serum
Decontaminate skin with 70% alcohol followed by iodine or chlorhexidine, collect at least 5 mL of blood into appropriate container
○ Collect stool immediately after bowel movement in a dry, clean container (e.g. Chamber pot, wide-mouth jar, pie plate, newspaper, or saran wrap on toilet seat). Follow instructions below to add specimen to BOTH yellow and white capped containers. Specimen should not be contaminated with urine or toilet water.
Container 1 SAF (Yellow top) vial:
○ Use plastic spork attached to lid to add sample (from beginning, middle and end of stool) to yellow capped bottle so that the preservative fluid level is approximately ¾ full. Mix thoroughly by shaking. Ratio should be 1 part solid stool to 3 parts SAF or 1 part liquid faeces to 1 part SAF.
Container 2 Sterile (White top) vial:
○ Using plastic spork, Add stool directly to sterile white capped container.
Sputum
○ Collect sputum by expectoration or by tracheal aspiration in sterile container.
Whole blood/serum
Decontaminate skin with 70% alcohol followed by iodine or chlorhexidine, collect at least 5 mL of blood into appropriate container
Refer to section Sample Processing / Delivery
PHOL
○ Microscopy: up to 5 days
○ Serology: up to 42 days
○ Serology: up to 42 days
416-586-4800 extension 4432
NO
○ Label specimen container, place in biohazard bag, seal and transport to the laboratory within 72 hours.
○ Specimens should be stored at room temp (20-25˚C) and transported to the UHN/SH Microbiology Laboratory ASAP after collection. For stool, keep Yellow top tube (SAF vial) at room temp while the White-top vial should be refrigerated (2-8˚C) prior to transport.
○ The specimen will be referred to the Public Health Ontario Laboratory for testing. PHOL will refer serology specimen to NRCP.
○ Specimens should be stored at room temp (20-25˚C) and transported to the UHN/SH Microbiology Laboratory ASAP after collection. For stool, keep Yellow top tube (SAF vial) at room temp while the White-top vial should be refrigerated (2-8˚C) prior to transport.
○ The specimen will be referred to the Public Health Ontario Laboratory for testing. PHOL will refer serology specimen to NRCP.