Parasites (other) – Trypanosoma
| Identification of Trypanosoma species or antibodies | |
| Microbiology | |
| Parasitology | |
| Serology ○ Whole blood ○ Serum Microscopy ○ Unstained thick and thin blood smear slides ○ EDTA blood (min volume 1 mL) ○ CSF ○ Lymph node aspirate PCR ○ EDTA blood |
Other Information
○ Infection by a blood parasite cannot be ruled out with a single blood sample. Acute and convalescent sera collected 2-3 weeks apart may be needed for laboratory diagnosis
○ Indicate relevant clinical information (e.g. recent travel history, recent refugee or immigrant/country of origin, fever, hemoglobinuria, intravascular hemolysis, renal failure or immune compromised)
○ Indicate relevant clinical information (e.g. recent travel history, recent refugee or immigrant/country of origin, fever, hemoglobinuria, intravascular hemolysis, renal failure or immune compromised)
Serology
○ Whole blood/serum: serum separator tube (clotted blood) (min volume 5 mL)
Microscopy/PCR
○ Unstained slides: Slide mailer
○ Whole blood: EDTA-blood tube (lavender top) (min volume 1 mL)
○ CSF/lymph node aspirate: sterile container
○ Whole blood/serum: serum separator tube (clotted blood) (min volume 5 mL)
Microscopy/PCR
○ Unstained slides: Slide mailer
○ Whole blood: EDTA-blood tube (lavender top) (min volume 1 mL)
○ CSF/lymph node aspirate: sterile container
Whole blood/serum
○ Decontaminate skin with 70% alcohol followed by iodine or chlorhexidine, collect at least 5 mL of blood in appropriate container
CSF
○ Decontaminate skin, collect at least 0.5 mL of CSF by lumbar puncture into sterile container
Lymph node aspirate
○ Decontaminate skin, collect lymph node aspirate via fine needle with sterile technique. Place in sterile container.
○ Decontaminate skin with 70% alcohol followed by iodine or chlorhexidine, collect at least 5 mL of blood in appropriate container
CSF
○ Decontaminate skin, collect at least 0.5 mL of CSF by lumbar puncture into sterile container
Lymph node aspirate
○ Decontaminate skin, collect lymph node aspirate via fine needle with sterile technique. Place in sterile container.
Refer to section Sample Processing / Delivery
PHOL
○ Microscopy: Up to 2 days
○ Serology/PCR: Up to 42 days
○ Serology/PCR: Up to 42 days
416-586-4800 extension 4432
NO
○ Label specimen container and outer biohazard specimen bag with printed electronic laboratory order sticker. For unstained blood slides, place in slide mailer. Place specimens in biohazard bag, seal.
○ Specimens should be stored at room temperature (20-25˚C) after collection and transported immediately to the UHN/SH Microbiology Laboratory
○ The specimen will be referred to the Public Health Ontario Laboratory for testing. PHOL will refer molecular and serologic testing to NRCP.
○ Specimens should be stored at room temperature (20-25˚C) after collection and transported immediately to the UHN/SH Microbiology Laboratory
○ The specimen will be referred to the Public Health Ontario Laboratory for testing. PHOL will refer molecular and serologic testing to NRCP.