Paroxysmal Nocturnal Hemoglobinuria

Alternative Names/Synonyms	PNH
Laboratory	Core Laboratory & Transfusion Medicine
Sub-section	Flow Cytometry
Specimen Type	Blood
Test Code	PNHFC

Ordering Instructions, Requisition

Mount Sinai Hospital -Special Laboratory Requisition Report Form MS 331

Clinical Significance

Flow cytometric PNH evaluation

Specimen Requirements (Sample Type)

Blood

Specimen Requirements (Volume (mL))

Optimum full draw

Specimen Requirements (Tube Type)

2 x EDTA Lavender Tube
Collection Containers – Tube Types

Collection Requirements

Blood Collection Instructions

Storage / Shipment Details

Room temperature

Sample Stability

Room temperature to be sent to testing hosipital within 24 hours of collection.

Cause for Rejection

Underfilled and clotted specimens and wrong anticoagulant.
Specimen Acceptability and Transport Guidance

Instrument, Methodology

UHN Flow Cytometry, Toronto General site

Test Availability

This test is referred out to another testing centre and is to be received in flow cytometry Monday to Friday and no later than 11:00 am on Fridays. No flow cytometry testing on weekend and statutory holidays. Specimen to be stored at room temperature.

Routine Turnaround Time

5 days

Reference Ranges

GPI-Deficient WBCs (FLAER-/CD157-):
CD15+ neutrophils: <0.1%
CD64+ monocytes: <0.1% In case of discrepancies, always interpret results using reference ranges as stated on laboratory reports and/or electronic patient chart.

GPI-Deficient WBCs (CD235a+/CD59-):
Type III RBCs: <0.01%
Type II RBCs: <0.01%
Totoal GPI-deficient RBCs: <0.01%

Laboratory Contact Information

416-586-4800 Extension 8519

Special Instructions

Diagnosis and/or indication of type of hematological pathology on the requistion will help with testing procedure.