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Phenotyping, Blood

Alternative Names/Synonyms	T and B Cell Phenotyping, Acute Leukemia Investigation, Multiple Myeloma Investigation, Lymphoma Investigation, NK Cell Investigation, Blood Flow Cytometry, Lymphoma protocol
Laboratory	Core Laboratory & Transfusion Medicine
Sub-section	Flow Cytometry
Specimen Type	Blood
Test Code	LYPNB

Ordering Instructions, Requisition

Mount Sinai Hospital -Special Laboratory Requisition Report Form MS 331, Womens College Hospital -Flow Cytometry Requisition Womens College Hospital, Markham Stouffville Hospital (Oak Valley Health) -Flow Cytometry Requisition Markham Stouffville

Clinical Significance

This test is used for preparation of a whole blood into a representative sample for introduction into the flow cytometer for immunophenotypic analysis.

Specimen Requirements (Sample Type)

Blood

Specimen Requirements (Volume (mL))

Optimum full draw

Specimen Requirements (Tube Type)

2 x EDTA Lavender Tube
Collection Containers – Tube Types

Collection Requirements

Blood Collection Instructions

Storage / Shipment Details

Room temperature

Sample Stability

To be processed within 48 hours of collection

Cause for Rejection

Samples that have the wrong anticoagulant or are clotted, or are greater than 48 hours old will be rejected. Specimens that are under-filled, hot or cold, may proceed with testing. However a comment will appear on the report to interpret results with caution.
Specimen Acceptability and Transport Guidance

Instrument, Methodology

Beckman Coulter Navios Ex

Test Availability

Monday to Friday and no later than 3 pm on Fridays. No Flow Cytometry testing on weekends and statutory holidays. Specimen to be stored at room temperature.

STAT Turnaround Time

STAT Acute Leukemia 24 hours

Routine Turnaround Time

4 days

Laboratory Contact Information

416-586-4800 Extension 8519

Special Instructions

Diagnosis and/or indication of type of hematological pathology on the requistion will help with testing procedure.

Ordering Practices

B and T cells flow panels are run and depening on results the hematopathologist may order follow up flow panels for further immunophenotypic investigation.