QuantiFERON-TB (QFT)

Alternative Names/Synonyms	Diagnosis of latent tuberculosis by detection of cell mediated immune response to peptide antigens that stimulate Mycobacterial proteins.
Laboratory	Microbiology
Sub-section	Bacteriology
Specimen Type	○ Whole blood

Other Information

○ This test is intended for use in conjunction with risk assessment, radiography and other medical and diagnostic evaluations.
○ A negative test does not rule out TB.

Specimen Requirements (Tube Type)

○ Whole blood: lithium-heparin tube (green top)

Collection Requirements

Whole blood:
○ Decontaminate skin with 70% alcohol followed by iodine or chlorhexidine, collect at least 5 mL of blood.

Sample Stability

Refer to section Sample Processing / Delivery

Referral Laboratory

Sick Kids ( plan to send to UHN in progress)

Routine Turnaround Time

10 to 14 days

Laboratory Contact Information

416-586-4800 extension 4618

Approval Required

Sample Processing / Delivery

○ Label specimen container. Place specimens in biohazard bag and seal.

○ Transport specimens to the UHN/SH Microbiology Laboratory at room temperature (20-25˚C). If a delay of more than 3 hours is expected, store at refrigeration temperature (2-8˚C). The specimen must first be held at room temperature for a minimum of 15 minutes.

○ The specimen will be referred to Sick Kids for testing.