Rubella – serology

Serologic detection of Rubella virus antibodies and avidity when clinically indicated
Microbiology
Serology
○ Whole blood ○ Serum
Other Information
○ If Rubella infection is suspected, collect additional samples for PCR testing. See Rubella – PCR

○ Rubella IgG testing will be performed by PHO laboratory on all requests for immunity serology (e.g. prenatal screening)
○ Rubella IgG and IgM testing will be performed on diagnostic requests with relevant clinical information
○ Collect the acute sample within 7 days after the onset of rash. Collect the convalescent 7-10 days after the acute.
○ For avidity testing, serum needs to be positive for Rubella IgG.
○ Serum separator tube (clotted blood)
○ Decontaminate skin with 70% alcohol followed by iodine or chlorhexidine, collect at least 5 mL of blood
○ Neonates: a minimum of 1 mL; if ordered as part of a TORCH screen, 2.5 mL of blood is sufficient for all serology tests

Refer to section Sample Processing / Delivery
In-house RUB Ig, PHOL - RUB IgM , RUB Avidity
○ Up to 5 days if sent to PHOL; 3 days if performed in house
○ Up to 14 days for avidity testing upon receipt by PHOL
416-586-4800 extension 4623
NO
○ Label specimen container. Place specimens in biohazard bag and seal.

○ Specimens should be stored and transported to the UHN/SH Microbiology Laboratory at refrigeration temperature (2-8˚C) after collection.

○ The specimen will be referred to the Public Health Ontario Laboratory for testing for IgM. IgG will be tested in house. PHOL will refer specimens for avidity testing to NML.