Sickle Cell Screen

Alternative Names/Synonyms	Hemoglobin S, HGB S, HB S, Sickledex, Sickle Prep, Sickle Solubility
Laboratory	Core Laboratory & Transfusion Medicine
Sub-section	Hematology
Specimen Type	Blood
Test Code	SKLSN

Ordering Instructions, Requisition

Hematopathology Requisition
(MS 333A)

Clinical Significance (If applicable)

Normal adult hemoglobin consists of Hb A (more than 95%) and Hb A2 (2.5 - 3.5%) and will demonstrate a negative sickle screen. Hemoglobin S is due to a structural variant of hemoglobin and demonstrates a positive sickle screen whether the patient is of the disease or of the trait variety. Note: This test should not be used for patients less than 6 months of age.

Specimen Requirements (Sample Type)

Whole Blood (EDTA)

Specimen Requirements (Volume (mL))

1 mL, test can be done on CBC tube, no extra tube is required if the CBC is ordered at the same time

Specimen Requirements (Tube Type)

Lavender whole blood tube with EDTA Collection Containers – Tube Types

Collection Requirements

Collection Instructions

Sample Stability

It may be used for 45 days when stored tightly capped at 2-8°C

Cause for Rejection

Samples from patients less than 6 months of age
Specimen Acceptability and Transport Guidance

Instrument, Methodology

Manual method - Sickle Solubility

Test Availability

24 hours, 7 days/week.

STAT Turnaround Time

1 hour

Routine Turnaround Time

2 hours

Reference Ranges

Negative In case of discrepancies, always interpret results using reference ranges as stated on laboratory reports and/or electronic patient chart.

Laboratory Contact Information

416-586-4800 Extension 4688

Ordering Practices

All patients positive for Sickle Screen will be set up for Hb Electrophoresis, if not previously.