Soft Tissue and Bone Biomarker Testing, Copy Number MDM2

Alternative Names/Synonyms	FISH, MDM2 FISH, MDM2 ISH, MDM2 Fluorescence in Situ Hybridization, CNV, Solid Tumour Biomarker Testing, Copy Number Variation, Sarcoma, Soft Tissue, Bone, MDM2, ISH
Laboratory	Anatomical Pathology
Sub-section	Surgical Pathology
Test Code	Group Test: MDM2GF

Ordering Instructions, Requisition

Pathology Consultation and Biomarker Requisition
https://www.mountsinai.on.ca/care/pathology/laboratory-forms-and-requisitions/pathology-consult-requisition

Other Information

In-house: Reflex test following 2+ HER2 IHC score.

Clinical Significance (If applicable)

Assessment of gene amplification (e.g. MDM2)
Biomarkers: FISH/Copy Number Variation
MDM2 FISH, where indicated, aids in classification, prognostic assessment, and therapeutic purposes.

Specimen Requirements (Sample Type)

Formalin fixed paraffin embedded block or tissue sections cut at 4 microns mounted on charged or coated slides.

Collection Requirements

Fixation between 6-72 hours

Storage / Shipment Details

Room temperature (block/unstained slide)

Cause for Rejection

Specimen Acceptability and Transport Guidance

Instrument, Methodology

Dako Omnis

References

Ontario Health: See Sarcoma Program

https://www.cancercareontario.ca/en/guidelines-advice/treatment-modality/pathology-laboratory-testing/genetic-testing-resources

Test Availability

Monday to Friday; 8 am - 4 pm

Routine Turnaround Time

Typically 7 days, with a maximum of 14 days

Reference Ranges

Please refer directly to the Pathology Report

Laboratory Contact Information

416-586-4800 Extension 4481 or 3813
Fax: (416) 586-8628
Email: PLMconsults@sinaihealth.ca

Ordering Practices

Typically used to query well-differentiated liposarcoma (WDLPS), atypical lipomatous tumour (ALT), or dedifferentiated liposarcoma (DDLPS).