○ Specimens positive by PCR are genotyped to identify azithromycin resistance in cases of suspected treatment failure
○ CSF: sterile container ○ Lesion/ulcer swab: Dacron swab in Universal Transport Medium ○ Whole blood: EDTA-blood tube (lavender top) ○ Fresh tissue: sterile container with a small amount of saline
CSF: ○ Decontaminate skin, perform lumbar puncture to collect at least 0.5 mL of CSF in sterile container
Lesion/genital ulcer swab ○ Cleanse the genital area by flushing with sterile physiological saline. Swab the base of the genital ulcer using a Dacron or cotton swab
Whole blood: ○ Decontaminate skin with 70% alcohol followed by iodine or chlorhexidine, collect at least 5 mL of blood
Tissue: ○ Collect at least 1 gram of tissue aseptically using surgical technique. Place in a clean, sterile container with a small amount of sterile saline.
Refer to section Sample Processing / Delivery
PHOL
○ 20 days
416-586-4800 extension 4631
YES
○ Label specimen container. Place specimens in biohazard bag and seal.
○ Specimens should be transported to the UHN/SH Microbiology Laboratory at refrigeration temperature (2-8˚C) ASAP after collection
○ The specimen will be referred to the National Microbiology Laboratory for testing.